Research Objectives – Antonelou Marianna

Research Objectives

Red cell biology in health and disease - RBC as biomarker of systemic state

Red cell storage lesion - Donor and Recipient variation effects on transfusion efficacy and outcomes

Extracellular vesicles

A. Publications

Red Cell biology in health and disease - RBC as biomarker of systemic states

The frequency of the allele αLELY, a low expression allele of the gene encoding erythroid spectrin α-chain, in the Greek population. Haematologica, 1999.
https://pubmed.ncbi.nlm.nih.gov/10457417/
A novel case of a haemoglobin H disease associated with clinical and morphological characteristics of congenital dyserythropoetic anaemia type I. Eur J Haematol, 68(4): 247-252, 2002.
https://doi.org/10.1034/j.1600-0609.2002.01590.x
Ultrastructural characterization of the erythroid cells in a novel case of congenital anaemia. Blood Cells Mol Dis, 2003.
https://doi.org/10.1016/S1079-9796(03)00006-8
Defective organization of the erythroid cell membrane in a novel case of congenital anemia. Blood Cells Mol Dis, 2003.
https://doi.org/10.1016/S1079-9796(03)00007-X
Physiologically important secondary modifications of red cell membrane in hereditary spherocytosis-evidence for in vivo oxidation and lipid rafts protein variations. Blood Cells Mol Dis, 2007.
https://doi.org/10.1016/j.bcmd.2006.10.163
Structural alterations of the erythrocyte membrane proteins in diabetic retinopathy. Graefe’s Arch Clin Exp Ophthalmol, 2007.
https://doi.org/10.1007/s00417-006-0500-6
Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex. Clin Cancer Res, 2009.
https://doi.org/10.1158/1078-0432.CCR-08-1805
Increased protein carbonylation of red blood cell membrane in diabetic retinopathy. Exp Mol Pathol, 2009.
https://doi.org/10.1016/j.yexmp.2009.04.001
Aging and death signaling in mature red cells: from basic science to transfusion practice. Blood Transfus, 2010.
https://doi.org/10.2450/2010.007S
Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis. J Proteomics, 2011.
https://doi.org/10.1016/j.jprot.2011.04.009
Apolipoprotein J/Clusterin is a novel structural component of human erythrocytes and a biomarker of cellular stress and senescence. PLoS ONE, 2011.
https://doi.org/10.1371/journal.pone.0026032
Blood modifications associated with end stage renal disease duration, progression and cardiovascular mortality: a 3-year follow-up pilot study. J Proteomics, 2014.
https://doi.org/10.1016/j.jprot.2014.02.009
Red blood cell abnormalities and the pathogenesis of anemia in end stage renal disease. Proteomics Clin Appl, 2016.
https://doi.org/10.1002/prca.201500127
Pathophysiological aspects of red blood cells in end-stage renal disease patients resistant to recombinant human erythropoietin therapy. Eur J Haematol, 2017.
https://doi.org/10.1111/ejh.12875
Short-term effects of hemodiafiltration versus conventional hemodialysis on erythrocyte performance. Can J Physiol Pharmacol, 2018.
https://doi.org/10.1139/cjpp-2017-0285
Coagulation abnormalities in renal pathology of chronic kidney disease: the interplay between blood cells and soluble factors. Biomolecules, 2021.
https://doi.org/10.3390/biom11091309
In Sickness and in Health: Erythrocyte Responses to Stress and Aging. Int J Mol Sci 2022.
https://doi.org/10.3390/ijms23136957

B. Publications

Red cell storage lesion - Donor and Recipient variation effects on transfusion efficacy and outcomes

Membrane protein carbonylation in non-leukodepleted CPDA-preserved red blood cells. Blood Cells Mol Dis, 2006.
https://www.sciencedirect.com/science/article/pii/S1079979606000337
Progressive oxidation of cytoskeletal proteins and accumulation of denatured hemoglobin in stored red cells. J Cell Mol Med, 2007.
https://doi.org/10.1111/j.1582-4934.2007.00008.x
Storage-dependent remodeling of the red blood cell membrane is associated with increased immunoglobulin G binding, lipid raft rearrangement and caspase activation. Transfusion, 2007.
https://doi.org/10.1111/j.1537-2995.2007.01254.x
Red blood cell aging markers during storage in citrate-phosphate-dextrose–saline-adenine-glucose-mannitol. Transfusion, 2010.
https://doi.org/10.1111/j.1537-2995.2009.02449.x
Aging and death signaling in mature red cells: from basic science to transfusion practice. Antonelou MH, Kriebardis AG, Papassideri IS. Blood Transfus, 2010.
https://doi.org/10.2450/2010.007S
Effects of pre-storage leukoreduction on stored red blood cells signaling: a time-course evaluation from shape to proteome. J Proteomics, 2012.
https://doi.org/10.1016/j.jprot. 2012.06.032
Uric acid variation among regular blood donors is indicative of red blood cells susceptibility to storage lesion markers: a new hypothesis tested. Transfusion, 2015.
https://doi.org/10.1111/trf.13211
An update on red blood cell storage lesions, as gleaned through biochemistry and omics technologies. Transfusion, 2015.
https://doi.org/10.1111/trf.12804
Donor variation effect on red blood cell storage lesion: a multi-parameter, yet consistent, story. Transfusion, 2016
https://doi.org/10.1111/trf.13582
Glucose 6-phosphate dehydrogenase deficient subjects may be better “storers” than donors of red blood cells. Free Radic Biol Med, 2016.
https://doi.org/10.1016/j.freeradbiomed. 2016.04.005
Data on how several physiological parameters of stored red blood cells are similar in glucose 6-phosphate dehydrogenase deficient and sufficient donors. Data Brief, 2016.
https://doi.org/10.1016/j.dib.2016.06.018
Donor-variation effect on red blood cell storage lesion: A close relationship emerges. Proteom Clin Appl, 2016.
https://doi.org/10.1002/prca.201500128
Insights into red blood cell storage lesion: toward a new appreciation. Antonelou MH, Seghatchian J. Transfus Apher Sci,2016.
https://doi.org/10.1016/j.transci.2016.10.019
Temperature-dependent haemolytic propensity of CPDA-1 stored erythrocytes vs. whole blood - Red cell fragility as a donor’s signature on blood units. Blood Transfus, 2017.
https://doi.org/10.2450/2017.0332-16
Unraveling the Gordian knot: red blood cell storage lesion and transfusion outcomes. Blood Transfus, 2017.
https://doi.org/10.2450/2017.0313-16
Red blood cell transfusion in surgical cancer patients: Targets, risks, mechanistic understanding and further therapeutic opportunities. Transfus Apher Sci, 2017.
https://doi.org/10.1016/j.transci.2017.05.015
Erythrocyte-based drug delivery in Transfusion Medicine: Wandering questions seeking answers. Transfus Apher Sci; 2017.
https://doi.org/10.1016/j.transci.2017.07.015
Hypoxia modulates the purine salvage pathway and decreases red blood cell and supernatant levels of hypoxanthine during refrigerated storage. Haematologica, 2018.
https://doi.org/10.3324/haematol.2017.178608
Donor-specific individuality of red blood cell performance during storage is partly a function of serum uric acid levels. Transfusion, 2018.
https://doi.org/10.1111/trf.14379
Metabolic linkage and correlations to storage capacity in erythrocytes from glucose 6-phosphate dehydrogenase deficient donors. Front Med, 2018.
https://doi.org/10.3389/fmed.2017.00248
Redox status, procoagulant activity and metabolome of fresh frozen plasma in glucose 6-phosphate dehydrogenase deficiency. Front Med, 2018.
https://doi.org/10.3389/fmed.2018.00016
Red cell transfusion in paediatric Red cell transfusion in paediatric patients with thalassaemia and sickle cell disease: Current status, challenges and perspectives. Transfus Apher Sci, 2018.
https://doi.org/10.1016/j.transci.2018.05.018
Recipient’s effects on stored red blood cell performance: the case of uremic plasma. Transfusion, 2019.
https://doi.org/10.1111/trf.15257
Ex vivo generation of transfusable red blood cells from various stem cell sources: A concise revisit of where we are now. Transfus Apher Sci, 2019.
https://doi.org/10.1016/j.transci.2018.12.015
“Valar morghulis”: all red cells must die. Blood Transfus, 2020.
https://doi.org/10.2450/2020.0028-20
When I need you most: frozen red blood cells for transfusion. Transfus Apher Sci, 2020.
https://doi.org/10.1016/j.transci.2020.102786
Sex-related aspects of the red blood cell storage lesion. Blood Transfus, 2021.
https://doi.org/10.2450/2020.0141-20
Red cell proteasome modulation by storage, redox metabolism and transfusion. Blood Transfus, 2022.
https://doi.org/10.2450/2020.0179-20
Beta-thalassemia minor is a beneficial determinant of red blood cell storage lesion. Haematologica, 2022.
https://doi.org/10.3324/haematol.2020.273946
Proteome of stored RBC membrane and vesicles from heterozygous beta thalassemia donors. Int J Mol Sci, 2021.
https://doi.org/10.3390/ijms22073369
Fatty acid desaturase activity in mature red blood cells and implications for blood storage quality. Transfusion, 2021.
https://doi.org/10.1111/trf.16402
Osmotic hemolysis is a donor-specific feature of red blood cells under various storage conditions and genetic backgrounds. Transfusion, 2021.
https://doi.org/10.1111/trf.16558
Red blood cell proteasome in beta-thalassemia trait: topology of activity and networking in blood bank conditions. Membranes, 2021.
https://doi.org/10.3390/membranes11090716
The post-storage performance of RBCs from beta-thalassemia trait donors is related to their storability profile. Int J Mol Sci, 2021.
https://doi.org/10.3390/ijms222212281
Corpuscular fragility and metabolic aspects of freshly drawn beta-thalassemia minor RBCs impact their physiology and performance post-transfusion: a triangular correlation analysis in vitro and in vivo. Biomedicines, 2022.
https://doi.org/10.3390/biomedicines10030530
Early and late-phase 24h responses of stored red blood cells to recipient-mimicking conditions. Front Physiol, 2022.
https://doi.org/10.3389/fphys.2022.907497
Innate variability in physiological and omics aspects of the beta thalassemia trait-specific donor variation effects. Front Physiol, 2022.
https://doi.org/10.3389/fphys.2022.907444
Supplementation with uric and ascorbic acid protects stored red blood cells through enhancement of non-enzymatic antioxidant activity and metabolic rewiring. Redox Biol, 2022.
https://doi.org/10.1016/j.redox.2022.102477
Tools and metrics for the assessment of post-storage performance of red blood cells: no one is left over. Transfusion, 2023.
https://doi.org/10.1111/trf.17228
The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation. Frontiers in Aging, 2023.

C. Publications

Extracellular vesicles

RBC-derived vesicles during storage. Ultrastructure, protein composition, oxidation and signalling components. Transfusion, 2008.
https://doi.org/10.1111/j.1537-2995.2008.01794.x
Apolipoprotein J/Clusterin in human erythrocytes is involved in the molecular process of defected material disposal during vesiculation. PLoS ONE, 2011.
https://doi.org/10.1371/journal.pone.0026033
Cell-derived microparticles in stored blood products: innocent-bystanders or effective mediators of post-transfusion reactions? Blood Transfus, 2012.
https://doi.org/10.2450/2012.006
Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects. Blood Transfus, 2016.
https://doi.org/10.2450/2016.0179-15
Update on extracellular vesicles inside red blood cell storage units: Adjust the sails closer to the new wind. Transfus Apher Sci, 2016.
https://doi.org/10.1016/j.transci.2016.07.016
The multi-faced extracellular vesicles in the plasma of chronic kidney disease patients. Front Cell Dev Biol, 2020.
https://doi.org/10.3389/fcell.2020.00227
Secretory phospholipase A2-IIA protein and mRNA pools in extracellular vesicles of bronchoalveolar lavage fluid from patients with early acute respiratory distress syndrome: A new perception in the dissemination of inflammation? Pharmaceuticals (Basel), 2020.
https://doi.org/10.3390/ph13110415
Proteome of stored RBC membrane and vesicles from heterozygous beta thalassemia donors. Int J Mol Sci, 2021.
https://doi.org/10.3390/ijms22073369
Leukoreduction makes a difference: a pair proteomics study of extracellular vesicles in red blood cell units. Transfus Apher Sci, 2021.
https://doi.org/10.1016/j.transci.2021.103166
Deciphering the relationship between free and vesicular hemoglobin in stored red blood cell units. Front Physiol, 2022.
https://doi.org/10.3389/fphys.2022.840995
Blood cell-derived microvesicles in hematological diseases and beyond. Biomolecules 12:803, 2022.
https://doi.org/10.3390/biom12060803

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