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PhD awarded
Awards
Projects
Methods
Research Objectives
Research team
Collaborators
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🏠
PhD awarded
Awards
Projects
Methods
Research Objectives
Research team
Collaborators
Contact us
Methods
Blood fractionation
Blood leukoreduction
Colorimetric Elisa
Confocal Laser Scanning Microscope (CLSM)
Conventional electron microscopy (TEM, SEM)
Density fractionation of RBCs (in vivo aging)
Flow cytometry
Fluorometry
Immunoblotting
Immunofluorescence microscopy
Immunogold electron microscopy (unicryl acrylic resins)
Immunoprecipitation
In vitro model of transfusion (RBC reconstitution with plasma at body ToC)
Isolation of RBCs and extracellular vesicles (EVs)
Subcellular fractionation of RBCs (preparation of ghosts, cytosol, membrane fractions/skeleton)
Statistical and network analyses
Assays for measurement of
Caspase activation (bulk, in situ)
EV-associated hemolysis
Hemolysis and cellular fragilities (upon storage, osmotic, mechanical and oxidative stresses)
Oxidative and Ca2+ stress (intracellular ROS/Ca2+ accumulation, protein oxidative modifications, lipid peroxidation)
Protein identification and quantitation (red cell fractions and EV phenotyping)
Proteasome activity
PS surface exposure
RBC immunophenotyping through multicolor FC
RBC shape distribution (spherocytosis, stomatocytosis, echinocytosis)
Total Antioxidant Capacity (TAC) of plasma (FRAP assay) (UA-dependent/independent)
Hereditary spherocytosis tests: cryohemolysis, Eosin-5-maleimide test, Acidified Glycerol Lysis test (AGLT50), osmotic resistance, SDS PAGE of RBC membrane proteins (Laemmli, Fairbanks, gel densitometry)
NKUA CORE FACILITIES (University Campus, Zografou)
Electron microscopy (SEM, TEM, Cryo)
Chromatography and Mass spectrometry (nano-LC, LC-MS/MS, MALDI-IMS-QTOF)
Next Generation Sequencing
NMR 500 ΜΗz
Raman spectroscopy
Oxidative status of Hb
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© Antonelou 2023
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University Campus, Zografou, Biology Build., 15784 Athens, Greece
manton@biol.uoa.gr
